Identifying Micro-Organisms

AIM: to practice using some sterile techniques, to interpret a range of micro-organisms found in lineage macroscopically and to examine a range of micro- organisms found in diet and water supply macroscopically APPARATUS:         Incubator          barren nutritive agar plates 1.         3 water ensamples 2.         1 air sample 3.         3 food samples          etna burner         Sterile pipettes          communicate loops         Sticky tape         Marking tape         Water samples(various)          yoghurt         Inoculating needle/ probe          sick from fruit, vegetables or cabbage         Methylated strong drink RISK ASSESMENT: 1.         Glassw are - bathroom cause cuts if handled incorrectly 2.         Bunsen burner - can c ause burn down if not on caoutchouc char 3.         Methylated enliven - toxic if ingested, highly combustible. Skin irritant 4.         Although the micro-organisms utilize in the activities in this manual are considered safe, standard microbiological appendage requires that they are regarded as potentially harmful SAFETY PROCEDURES: 1.         Handle with care, do not set out alone glassware near the bounds of the bench 2.         take care, do not leave flame unattended keep flammable liquid i.e. Methylated pot liquor away 3.         Keep Methylated spirits away from flames. take over gloves when handling it 4.         Wear gloves, lab coat and safety glasses. all used equipment should be placed in disinfectant.

Dispose of cultures by and by use, in an autoclave. Wash hands forrader leaving lab. neer open agar plate after setting up METHOD: 1.         Sterilise the workbench area by mopping alcohol on to it 2.         collect 8 sterile food for thought agar plates 3.         A sample of honeyed milk was obtained 4.         leave one plate aside, certain and taped as the sway 5.          use a sterile inoculating loop, a subtle sample of the fresh milk was streaked onto the plates. This plate was consequently sealed with sticky tape and labeled as fresh milk 6.          travel 3 and 4 were then retell with tap water, distilled water, seek tank water, fresh milk, old milk, If you lack to get a full essay, order it on our website: OrderEssay.net

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